Immunochemical studies on the phase variations of Bordetella pertussis. by Marie Ann Aprile

Cover of: Immunochemical studies on the phase variations of Bordetella pertussis. | Marie Ann Aprile

Published in [Toronto] .

Written in English

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Subjects:

  • Bordetella pertussis.,
  • Immunochemistry.,
  • Whooping cough -- Immunological aspects

Edition Notes

Book details

ContributionsToronto, University.
The Physical Object
Pagination190 leaves.
Number of Pages190
ID Numbers
Open LibraryOL21625624M

Download Immunochemical studies on the phase variations of Bordetella pertussis.

Studies on phase variation in Bordetella pertussis. Goldman S, Hanski E, Fish F. Pathogenic strains of Bordetella pertussis undergo spontaneous phase variation and become non-pathogenic Cited by: 1. Cite this article. Stibftz, S., Aaronson, W., Monackt, D. et al. Phase variation in Bordetella pertussis by frameshift mutation in a gene for a novel two-component Cited by: Lipopolysaccharide (LPS) was extracted from three Phase I strains (,and ) and one Phase IV strain () of was also obtained from ATCC and NCTC which are listed as sis Phase IV but proved to be Lophomonas sp.

and Brucella melitensis type 1. Chromatographic studies and chemical analysis revealed that all four by:   Bordetella (B.) pertussis resurgence affects not only the unvaccinated, but also the vaccinated population. Different vaccines are available, however, it is currently unknown whether the type of childhood vaccination has an influence on antibody responses following a B.

pertussis infection later in life. Therefore, the study aim was to profile serum antibody responses in young adults with Cited by: 5. Murgo, A.J., and Athanassiades, T.J.,Studies on the adjuvant effect of Bordetella pertussis vaccine to sheep erythrocytes in the mouse.

In vitro enhancement of antibody formation with normal spleen cells, J. : Stephen I. Morse. Bordetella pertussis is a Gram-negative bacterium that causes pertussis or whooping cough in humans.

The incidence of pertussis has increased in young infants, adolescents and adults in many countries where the vaccination against pertussis has been used extensively.

Bordetella are Gram-negative bacteria that colonize ciliated respiratory epithelial surface of variety of hosts. Whooping cough is an acute respiratory disease caused by the bacterium Bordetella pertussis.B.

pertussis expresses several factors that contribute to its ability to cause disease. Several of these factors, including filamentous hemagglutinin, pertactin, fimbriae, and tracheal.

Early studies showed that homopolymeric tracks (HPTs) of Cs result in phase variation in B. pertussis. Insertion of a C in a HPT of 6 Cs in the gene coding for the sensor component (bvgS) of a two-component sensory transduction system of B. pertussis, resulted in.

1. Pertussis. Bordetella pertussis is a gram-negative bacterium that causes the serious respiratory disease whooping cough, or pertussis. Often considered a disease affecting only children, it is now clear that all ages are affected but young infants are at greatest risk of severe pertussis as a result of their immature respiratory systems and being too young to have received the full course.

Pertussis 16 Pertussis, or whooping cough, is an acute infectious disease caused by the bacterium Bordetella pertussis. Outbreaks of pertussis were first described in the 16th century, and the organism was first isolated in In the 20th century, pertussis was one of the most common childhood diseases and a major cause of childhood.

Bordetella pertussis causes whooping cough (“le Microbe de la coqueluche”). Despite extensive molecular studies, whole- genome sequencing, detailed protein characterization, and gene expression studies, much remains unknown about this microbe.

Effective pertussis whole-cell (Pw) vaccines have been used to vaccinate infants and toddlers. Amodel ofthe molecularbasis ofthe phase regulation is presented.

Bordetella pertussis strains undergo a form of variation, often called a change of phase, in which virulent bacteria simultaneouslylosetheability to synthesizetoxinsandother factors associated with pathogenicity (7, 20). These factors include pertussis toxin (15), adenylate.

Knowledge of Bordetella pertussis, ‘Le microbe de la coqueluche’ (the whooping cough germ), is over years old.A member of the Bordetella genus, it is a Gram-negative, aerobic, non-motile bacterium and is the cause of a highly contagious respiratory disease in humans.

Several reviews have been written recently about this bacterium and the human disease it is associated with (such as. These studies varied in type and number of vaccines, design, case definition, and laboratory method used to confirm the diagnosis of pertussis, so comparison among studies must be made with caution.

Point estimates of vaccine efficacy ranged from 80% to 85% for vaccines currently licensed in. The current resurgence of whooping cough is alarming, and improved pertussis vaccines are thought to offer a solution.

Outer membrane vesicle vaccines (omvPV) are potential vaccine candidates, but omvPV-induced humoral responses have not yet been characterized in detail. The purpose of this study was to determine the antigen composition of omvPV and to elucidate the immunogenicity of the.

phenomenon was called phase variation, and it is rarely reversible (34). So far, all characterized spontaneous phase variants havebeenshownto contain small deletions orframe-shift mutations in thebvgSgene (3).

Recently, a modelfor the virulence regulation in B. pertussis wasproposed, in which the BvgS sensor protein perceives external stimuli. We shall repeat the present studies with isogenic pairs of phase variants [10,15] to more precisely define the association of DNA rearrangements detected with the repeated DNA sequence probe to the phase variation of B.

pertussis. 1 1 jp B BC _g EL I. Data to compare detection of Bordetella pertussis by qPCR and culture at Days 0– These data are derived from the 15 participants who were inoculated with 10 5 colony forming units of Bordetella pertussis.

Pernasal swabs were taken on Days 3, 5, 7, 9, 11, and Anderson P, Flesher A, Shaw S, Harding AL, Smith DH. Phenotypic and genetic variation in the susceptibility of Haemophilus influenzae type b to antibodies to somatic antigens. J Clin Invest.

Apr; 65 (4)– [PMC free article] Aprile MA, Wardlaw AC. Immunochemical studies on the lipopolysaccharides of Bordetella pertussis. The effect of the addition of (2,6-O-dimethyl)-β-cyclodextrin (MeβCD) during growth of Bordetella pertussis in synthetic Stainer-Scholte liquid medium (SS) on lipopolysaccharide (LPS; endotoxin) release was investigated.

The MeßCD concentration used (3 mg/ml) was chosen according to the optimal level found in previous studies to enhance major soluble antigen production.

Molecular Pathogenesis of Bordetella pertussis M. Madan Babu, 1* J. Bhargavi, 1 Ranajeet Singh Saund, 1 and S. Kumar Singh 2 1 Center for Biotechnology, Anna University, Chennai2 Molecular Biophysics Unit, Indian Institute of Science, Bangalore Abstract. Bordetella pertussis is an aerobic, non-spore forming gram negative coccobacillus that colonizes the respiratory.

Pertussis is a severe respiratory infection caused by Bordetella pertussis, and inpertussis was associated with an estimated 16 million cases anddeaths globally. Sizeable outbreaks of pertussis have been reported over the past 5 years, and disease reemergence has been the focus of international attention to develop a deeper understanding of pathogen virulence and genetic.

In summary, the LOS of B. pertussis lacks the long-chain polysaccharide O antigen, and is resolved electrophoretically into two distinct bands designated LOS A and LOS B. Mice administered with anti-LOS A mAbs can be protected from fatal B.

pertussis infection. These observations may indicate potential for oligosaccharides as a component of new acellular pertussis vaccines. Goldman, S, Navon, Y, and Fish, F () Phase variation in Bordetella pertussis is accompanied by changes in DNA modification. Microbial Pathogenesis 2, – Microbial Pathogenesis 2, –   Background Acellular pertussis vaccines do not control pertussis.

A new approach to offer protection to infants is necessary. BPZE1, a genetically modified Bordetella pertussis strain, was developed as a live attenuated nasal pertussis vaccine by genetically eliminating or detoxifying 3 toxins. Methods We performed a double-blind, placebo-controlled, dose-escalating study of BPZE1 given.

Acellular vaccines against Bordetella pertussis were introduced in Australia in Bythese vaccines had replaced whole-cell vaccines. During –, a large outbreak of pertussis occurred. During this period, 30% (96/) of B. pertussis isolates did not express the vaccine antigen pertactin (prn). Multiple mechanisms of prn inactivation were documented, including IS and.

Bacterial Adhesion Bacterial Inoculum Yersinia Enterocolitica Bordetella Pertussis Microbial Adhesion These keywords were added by machine and not by the authors.

This process is experimental and the keywords may be updated as the learning algorithm improves. Aprile MA, Wardlaw AC. Immunochemical studies on the lipopolysaccharides of Bordetella pertussis. Can J Microbiol. Feb; 19 (2)– Aprile MA, Wardlaw AC.

Availability and specificity of lipopolysaccharide on the surface of 56 degrees C heated Bordetella pertussis. Can J Microbiol.

Apr; 19 (4)– The decision to treat a suspected case of pertussis with antibiotics is usually based on a clinical diagnosis rather than waiting for laboratory confirmation. The current guideline focuses on making the clinical diagnosis of pertussis-associated cough in adults and children.

Bordetella pertussis causes the disease whooping cough through coordinated control of virulence factors by the Bordetella virulence gene system. Microarrays and, more recently, RNA sequencing (RNA-seq) have been used to describe in vitro gene expression profiles of B.

pertussis and other pathogens. In previous studies, we have analyzed the in vitro gene expression profiles of B.

pertussis. The monoclonal antibodies might be useful for the detection of soluble antigens and whole bacteria in clinical samples and for studies of the immunochemical structure of B.

pertussis LPS. Full text Get a printable copy (PDF file) of the complete article (M), or click on a. Aprile MA, Wardlaw AC. Immunochemical studies on the lipopolysaccharides of Bordetella pertussis.

Can J Microbiol. Feb; 19 (2)– Archambault D, Rondeau P, Martin D, Brodeur BR. Characterization and comparative bactericidal activity of monoclonal antibodies to Bordetella pertussis lipo-oligosaccharide A. J Gen Microbiol. AIM: In pertussis-like respiratory infections, once pertussis has been laboratory confirmed, other potential causative pathogens will seldom be looked for.

Probably most mixed infections are found accidentally and since these mixed infections might cause a more severe disease we performed a retrospective study of their incidence.

SUMMARY Bordetella respiratory infections are common in people (B. pertussis) and in animals (B. bronchiseptica). During the last two decades, much has been learned about the virulence determinants, pathogenesis, and immunity of Bordetella.

Clinically, the full spectrum of disease due to B. pertussis infection is now understood, and infections in adolescents and adults are recognized as.

with the emergence of Bordetella pertussis strains carrying a novel allele for the pertussis toxin promoter, which confers increased pertussis toxin (Ptx) production. Epidemiologic data suggest that these strains are more virulent in humans.

We discuss changes in the ecology of B. pertussis that may have driven this adaptation. Bordetella pertussis, the pertussis agent, secretes an adenylate cyclase toxin-hemolysin (CyaA) that binds myeloid phagocytes through complement receptor 3 (CD11b/CD18) and swiftly delivers its.

Bordetella pertussis causes whooping cough or pertussis, a respiratory disease that is most severe in infants. Before childhood vaccination was introduced in the s, pertussis was a major cause of infant deaths worldwide. Widespread vaccination of children reduced the incidence of illness and deaths caused by pertussis ().However, globally pertussis remains 1 of the top 10 causes of death.

Background. Recent studies worldwide have reported increasing numbers of adults diagnosed with Bordetella pertussis despite receiving childhood vaccinations. This study describes a pertussis outbreak at a university medical faculty campus and examines the effectiveness of diphtheria, tetanus, and pertussis (DTaP) vaccination completed during infancy in Japan.

Betsy Kuipers's 63 research works with 1, citations and 3, reads, including: MenB‐4C vaccine induces effective opsonophagocytic killing in children with a complement deficiency.

Despite vaccination since the s, pertussis has persisted and resurged. It remains a major cause of infant death worldwide and is the most prevalent vaccine-preventable disease in developed countries.

The resurgence of pertussis has been associated with the expansion of Bordetella pertussis strains with a novel allele for the pertussis toxin (Ptx) promoter, ptxP3, which have. Abstract. C1 esterase inhibitor (C1inh) is a major inhibitor of several pathways of inflammation in humans.

In this study, we show that virulent-phase cultures of Bordetella pertussis, the etiological agent for whooping cough, but not other Bordetella species specifically recruit C1inh from human serum. Using a spontaneous mutant of B.

pertussis that was deficient in C1inh binding, we.by immunochemical confirmation! Bordetella pertussis,!B. parapertussis! B. bronchiseptica! Y. entercolitica,!

Y. pseudotuberculosis! Burkholderia (including B. mallei)! Stenotrophomonas! Klebsiella Shigella! Group B streptococcus! S. pneumoniae! Vibrio cholerae! Enterococcus faecalis!

.The B. pertussis lep gene has recently been cloned and characterised, and future studies may determine whether Lep is an absolute requirement for FHA secretion in B. pertussis. The C-terminal third of the precursor protein is also removed leaving mature FHA comprising the N-terminal kDa of FhaB.

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